Cell envelope diversity and evolution across the bacterial tree of life

Hashimi A and Elitza I. Tocheva
Nat Microbiology. 2024 Oct 18; doi: 10.1038/s41564-024-01812-9

The diversity of clinical Mycobacterium abscessus isolates in morphology, glycopeptidolipids and infection rates in a macrophage model

Pichler V, Dalkilic L, Shoaib G, Shapira T, Rankine-Wilson L, Boudehen Y-M, Chao JD, Sexton DL, Prieto M, Quon BS, Tocheva EI, Kremer L, Hsiao W and Av-Gay Y
J Med Microbiol. 2024 Aug; doi: 10.1099/jmm.0.001869

The cell envelope of Mycobacterium abscessus and its role in pathogenesis

Parmar S and Tocheva EI
PLoS Pathogens. 2023 May 18; doi.org/10.1371/journal.ppat.1011318

The cell envelope architecture of Deinococcus: HPI forms the S- layer and SlpA tethers the outer membrane to peptidoglycan

Bharat TAM, Tocheva EI and Alva V
PNAS. 2023 Dec 19; doi: 10.1073/pnas.2305338120

The cell envelope of Thermotogae suggests a mechanism for outer membrane biogenesis

Danielle L. Sexton, Ameena Hashimi, Polina Beskrovnaya, Lloyd Sibanda, Tao Huan, Elitza I. Tocheva
Proc Natl Acad Sci. 2023 Apr 24; 120(18) doi.org/10.1073/pnas.2303275120

Interdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacterium Deinococcus radiodurans

von Kügelgen A, van Dorst S, Yamashita K, Sexton DL, Tocheva EI, Murshudov G, Alva V, Bharat TAM
Prot Natl Acad Sci. 2023 Apr 12:120(16) doi.org/10.1073/pnas.2215808120

Thermophilic Dehalococcoidia with unusual traits shed light on an unexpected past

Palmer M, Covington J, Zhou E, Thomas S, Habib N, Seymour C, Lai D, Johnston J, Jiao J, Muok A, Dodsworth J, Liu L, Xian W, Zhi X, Silva L, Bowen B, Louie K, Briegel A, Tocheva EI, Woyke T, Northen T, Weber P, Pett-Ridge J, Mayali X, Li W, Hedlund B. ISME J. 2023 Apr 11: doi.org/10.1038/s41396-023-01405-0

Cryptosporulation in Kurthia spp. forces a rethinking of asporogenesis in Firmicutes

Fatton M, Filippidou S, Junier T, Cailleau G, Berge M, Poppleton D, Blum T, Kaminek M, Odriozola A, Blom J, Johnson S, Abrahams J, Chain P, Gribaldo S, Tocheva EI, Zuber B, Viollier P, Junier P.
Environ Microbiol. 2022 July 20; DOI: 10.1111/1462-2920.16145.

Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans

Danielle L. Sexton, Steffen Burgold, Andreas Schertel, Elitza I. Tocheva
Curr Res Struct Biol. 2021 Dec 13;4:1-9. doi: 10.1016/j.crstbi.2021.12.001.

Ultrastructure of organohalide-respiring Dehalococcoidia revealed by cryo-electron tomography

Danielle L. Sexton, Gao Chen, Fadime Kara Murdoch, Ameena Hashimi, Frank E. Löffler, Elitza I. Tocheva
Appl Environ Microbiol. 2021 Nov 17;AEM0190621. doi: 10.1128/AEM.01906-21.

Loss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis

Mohammed Kaplan, Elitza I Tocheva, Ariane Briegel, Megan J Dobro, Yi-Wei Chang, Poorna Subramanian, Alasdair W McDowall, Morgan Beeby, Grant J Jensen
mBio https://journals.asm.org/doi/10.1128/mBio.00298-21

Structural, Metabolic and Evolutionary Comparison of Bacterial Endospore and Exospore Formation

Polina Beskrovnaya, Danielle L Sexton, Mona Golmohammadzadeh, Ameena Hashimi, Elitza I Tocheva
Front Microbiol https://www.frontiersin.org/articles/10.3389/fmicb.2021.630573/full

Roles for phthiocerol dimycocerosate lipids in Mycobacterium tuberculosis pathogenesis

Céline Rens, Joseph D Chao, Danielle L Sexton, Elitza I Tocheva, Yossef Av-Gay
Microbiology https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001042

Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples

Maheux AF, Boudreau DK, Abed JY, Bérubé E​, Brodeur S, Bernard KA, Hashimi A​, Ducrey E, Guay EF, Raymond F, Corbeil J, Domingo MC, Roy PH, Boissinot M, Tocheva EI, Omar RF
IJSEM https://doi.org/10.1099/ijsem.0.004691

No endospore formation confirmed in members of the phylum Proteobacteria

Beskrovnaya P, Fakih D, Morneau I, Hashimi A, Guadarrama Bello D, Xing S, Nanci A, Huan T, Tocheva EI
Applied and Environ Microbiol. 2020, doi:10.1128/AEM.02312-20

Structure of the bacterial cellulose ribbon and its assembly-guiding cytoskeleton by electron cryotomography

Nicolas WJ , Ghosal D , Tocheva EI , Meyerowitz EM , Jensen GJ
Journal of Bacteriology. 2020, Nov. 16; doi: 10.1128/JB.00371-20.

Ultrastructure of Exospore Formation in Streptomyces Revealed by Cryo-Electron Tomography

Sexton DL and Tocheva EI.
Frontiers in Microbiology. 2020, Sept. 24; doi: 10.3389/fmicb.2020.581135.

Bacterial flagellar motor PL-ring disassembly subcomplexes are widespread and ancient

Kaplan M, Sweredoski MJ, Rodrigues JP, Tocheva EI, Chang YW, Ortega DR, Beeby M, Jensen GJ.
Proc Natl Acad Sci U S A. 2020, Apr 21; 117(16):8941-8947. doi: 10.1073/pnas.1916935117.

De novo structural pattern mining in cellular electron cryotomograms

Xu M, Singla J, Tocheva EI, Chang YW, Stevens RC, Jensen GJ, Alber F.
Structure, 2019, April 2, 27(4):679-691.e14. doi: 10.1016/j.str.2019.01.005.

The Mycobacterial Cell Envelope: A Relict From the Past or the Result of Recent Evolution?

Vincent AT, Nyongesa S, Morneau I, Reed MB, Tocheva EI, Veyrier FJ.
Front Microbiol, 2018, Oct 9;9:2341. doi: 10.3389/fmicb.2018.02341.

Uncharacterized bacterial structures revealed by electron cryotomography

Dobro MJ, Oikonomou CM, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis BA, Kjær A, Jewett AI, McDowall AW, Chen S, Chang YW, Shi J, Subramanian P, Iancu CV, Li Z, Briegel A, Tocheva EI, Pilhofer M, Jensen GJ.

J Bacteriol. 2017 Jun 12. pii: JB.00100-17. doi: 10.1128/JB.00100-17.

Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa

Lisa R. Racki, Elitza I. Tocheva, Michael G. Dieterle, Meaghan C. Sullivan, Grant J. Jensen, and Dianne K. Newman
PNAS 2017 ; published ahead of print March 6, 2017, doi:10.1073/pnas.1615575114

Starvation and recovery in the deep-sea methanotroph Methyloprofundus sedimenti.

Tavormina PL, Kellermann MY, Antony CP, Tocheva EI, Dalleska NF, Jensen AJ, Valentine DL, Hinrichs KU, Jensen GJ, Dubilier N, Orphan VJ.
Mol Microbiol. 2017 Jan;103(2):242-252. doi: 10.1111/mmi.13553.
PMID:27741568

Sporulation, bacterial cell envelopes and the origin of life

Tocheva EI, Ortega DR, Jensen GJ.
Nat Rev Microbiol, 2016 Aug;14(8):535-542. doi: 10.1038/nrmicro.2016.85.

Streptomyces: a screening tool for bacterial cell division inhibitors.

Jani C, Tocheva EI, McAuley S, Craney A, Jensen GJ, Nodwell J.
J Biomol Screen. 2015 Feb;20(2):275-84. doi: 10.1177/1087057114551334.
PMID: 25256667

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography.

Chang YW, Chen S, Tocheva EI, Treuner-Lange A, Löbach S, Søgaard-Andersen L, Jensen GJ.
Nat Methods. 2014 Jul;11(7):737-9. doi: 10.1038/nmeth.2961.
PMID: 24813625

Structure and expression of propanediol utilization microcompartments in Acetonema longum.

Tocheva EI, Matson EG, Cheng SN, Chen WG, Leadbetter JR, Jensen GJ.
J Bacteriol. 2014 May;196(9):1651-8. doi: 10.1128/JB.00049-14.
PMID: 24532773

Polyphosphate storage during sporulation in the gram-negative bacterium Acetonema longum.

Tocheva EI, Dekas AE, McGlynn SE, Morris D, Orphan VJ, Jensen GJ.
J Bacteriol. 2013 Sep;195(17):3940-6.
PMID: 23813732

Peptidoglycan transformations during Bacillus subtilis sporulation.

Tocheva EI, López-Garrido J, Hughes HV, Fredlund J, Kuru E, Vannieuwenhze MS, Brun YV, Pogliano K, Jensen GJ.
Mol Microbiol. 2013 May;88(4):673-86. doi: 10.1111/mmi.12201.
PMID: 23531131

Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation.

Tocheva EI, Matson EG, Morris DM, Moussavi F, Leadbetter JR, Jensen GJ.
Cell. 2011 Sep 2;146(5):799-812. doi: 10.1016/j.cell.2011.07.029.
PMID: 21884938

Nanopods: a new bacterial structure and mechanism for deployment of outer membrane vesicles.

Shetty A, Chen S, Tocheva EI, Jensen GJ, Hickey WJ.
PLoS One. 2011;6(6):e20725. doi: 10.1371/journal.pone.0020725.
PMID: 21687732

Structural diversity of bacterial flagellar motors.

Chen S, Beeby M, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Müller A, Dobro MJ, Jensen GJ.
EMBO J. 2011 Jun 14;30(14):2972-81. doi: 10.1038/emboj.2011.186.
PMID: 21673657

Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria.

Swulius MT, Chen S, Jane Ding H, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, Jensen GJ.
Biochem Biophys Res Commun. 2011 Apr 22;407(4):650-5. doi: 10.1016/j.bbrc.2011.03.062.
PMID: 21419100

Electron cryotomography.

Tocheva EI, Li Z, Jensen GJ.
Cold Spring Harb Perspect Biol. 2010 Jun;2(6):a003442. doi: 10.1101/cshperspect.a003442. Review.
PMID: 20516135

Electron cryotomography of bacterial cells.

Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJ.
J Vis Exp. 2010 May 6;(39). pii: 1943. doi: 10.3791/1943.
PMID: 20461053

Universal architecture of bacterial chemoreceptor arrays.

Briegel A, Ortega DR, Tocheva EI, Wuichet K, Li Z, Chen S, Müller A, Iancu CV, Murphy GE, Dobro MJ, Zhulin IB, Jensen GJ.
Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17181-6. doi: 10.1073/pnas.0905181106.
PMID: 19805102

A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.

Dresen C, Lin LY, D’Angelo I, Tocheva EI, Strynadka N, Eltis LD.
J Biol Chem. 2010 Jul 16;285(29):22264-75. doi: 10.1074/jbc.M109.099028.
PMID: 20448045

Conserved active site residues limit inhibition of a copper-containing nitrite reductase by small molecules.

Tocheva EI, Eltis LD, Murphy ME.
Biochemistry. 2008 Apr 15;47(15):4452-60. doi: 10.1021/bi7020537.
PMID: 18358002

Stable copper-nitrosyl formation by nitrite reductase in either oxidation state.

Tocheva EI, Rosell FI, Mauk AG, Murphy ME.
Biochemistry. 2007 Oct 30;46(43):12366-74.
PMID: 17924665

Effect of the methionine ligand on the reorganization energy of the type-1 copper site of nitrite reductase.

Wijma HJ, MacPherson I, Farver O, Tocheva EI, Pecht I, Verbeet MP, Murphy ME, Canters GW.
J Am Chem Soc. 2007 Jan 24;129(3):519-25.
PMID: 17227014

Structures of ternary complexes of BphK, a bacterial glutathione S-transferase that reductively dechlorinates polychlorinated biphenyl metabolites.

Tocheva EI, Fortin PD, Eltis LD, Murphy ME.
J Biol Chem. 2006 Oct 13;281(41):30933-40.
PMID: 16920719

Side-on copper-nitrosyl coordination by nitrite reductase.

Tocheva EI, Rosell FI, Mauk AG, Murphy ME.
Science. 2004 May 7;304(5672):867-70.
PMID: 15131305

A caged lanthanide complex as a paramagnetic shift agent for protein NMR.

Prudêncio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M.
Chemistry. 2004 Jul 5;10(13):3252-60.
PMID: 15224334